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A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination. There is a clear distinction between serum-free media and chemically defined media. Serum-free media may contain undefined animal-derived products such as serum albumin (purified from blood), hydrolysates, growth factors, hormones, carrier proteins, and attachment factors. These undefined animal-derived products will contain complex contaminants, such as the lipid content of albumin. In contrast, chemically defined media require that all of the components must be identified and have their exact concentrations known. Therefore, a chemically defined medium must be entirely free of animal-derived components and cannot contain either fetal bovine serum, bovine serum albumin or human serum albumin. To achieve this chemically defined media is commonly supplemented with recombinant versions of albumin and growth factors, usually derived from rice or ''E. coli'', or synthetic chemical such as the polymer polyvinyl alcohol which can reproduce some of the functions of BSA/HSA. The constituents of a chemically defined media include: a basal media (such as DMEM, F12, or RPMI 1640, containing amino acids, vitamins, inorganic salts, buffers, antioxidants and energy sources), which is supplemented with recombinant albumin, chemically defined lipids, recombinant insulin and/or zinc, recombinant transferrin or iron, selenium and an antioxidant thiol such as 2-mercaptoethanol or 1-thioglycerol. ==The advantages of chemically defined media== * Avoidance of batch to batch variation of bovine serum or albumin, which causes inconsistency in growth-promoting properties. * Low protein content, which can hinder product purification. * Elimination of the risk of contaminants—viruses, mycoplasma, prions from animal-derived products which may be transmitted to the end product used by humans, e.g., bovine spongiform encephalopathy (BSE) or mad cow disease. * Elimination of factors that may interfere with hormones or growth factors when studying their interaction with cells. * Removal of concerns regarding the limited availability of fetal bovine serum, with periods of world shortages. * Reduced cost: fetal calf serum can account for up to 85% of the over-all cost of the medium when calculated for large-scale cultures. * There are increasing concerns about animal suffering inflicted during serum collection that add an ethical imperative to move away from the use of serum wherever possible. Chemically defined media also allows researchers who are studying in the field of cell physiology (especially extracellular) and or molecule–cell interactions to eliminate any variables that may arise due to the effects of unknown components in the medium. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Chemically defined medium」の詳細全文を読む スポンサード リンク
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